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Verification of the binding of RifZ-5 to the promoter regions of rifK and orf18 by DNase I footprinting assay. rifK (A) and orf18 (B) were identified through searching with the RifZ box in U32 at the whole-genome scale. The DNase I footprinting assay further confirmed that RifZ could specifically bind to both promoter regions, and the two RifZ-5-protected DNA sequences contained the RifZ binding motif, which is highlighted in red.
