Fig. 3.

Central mitosomes divide during prophase and associate with G. intestinalis karyomastigont. a Mitosomes of mitotic cells were immunolabeled with an anti-GL50803_9296 antibody. The division of the central mitosome could be observed only during prophase as a prominent V-shaped arrangement before the segregation of the daughter kinetosomes and chromosomes. b G. intestinalis cells expressing C-terminally human influenza hemagglutinin (HA)-tagged centrin were enriched for mitotic cells by albendazole treatment. The cells were fixed and immunolabeled with anti-HA (green) and anti-GL50803_9296 antibodies (red). The nuclei were stained with DAPI (blue). Arrowheads indicate separation of the central mitosomes coupled with division of the basal bodies. Scale bars, 2 μm. c Expression of the synthetic linker composed of the outer mitosomal membrane protein GiMOMP35 and GiQb4 SNARE protein induces aggregation of the mitosomes to the cell periphery and the formation of the endoplasmic reticulum–mitosome chimeras. The cells were fixed and immunolabeled with anti-HA (green), anti-GL50803_9296 antibodies (red), and anti-PDI2 antibodies (magenta). The nuclei were stained with DAPI (blue). DIC differential interference contrast microscopy