Figure 3.

Changes to myocardial ultrastructure, observed using electron microscopy (magnification, 1,300,000x). (A) Non-ischemic control group; muscle fibers were correctly arranged, the sensitive sectors were clear, and the outer mitochondrial membrane and crests were complete. (B) Ischemic control group; muscle fibers were disordered, the mitochondrial crests were reduced and the outer mitochondrial membranes were damaged. (C) Remifentanil-treated group; muscle fibers were correctly arranged and rich in mitochondria, and the mitochondrial crests and outer membranes had retained their integrity. (D) Naloxone-treated group; muscle fibers were disorganized, the mitochondrial crests were reduced and vacuoles were degenerated. (E) Remifentanil and naloxone co-treated group; cardiac lines were blurred, and the mitochondrial crests and outer membranes were defective. The white arrows indicate the mitochondria and mitochondrial cristae of the myocardial cells.