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. 2017 Feb 10;13(4):1506–1513. doi: 10.3892/etm.2017.4115

Figure 1.

Figure 1.

Efficiency of knockdown and overexpression of USP39 in SMMC-7721 cells. (A) The expression of USP39 mRNA relative to the expression of internal control protein GADPH in SMMC-7721/NC, SMMC-7721/KD and SMMC-7721/KD# cells was examined with reverse transcription-quantitative polymerase chain reaction. (B) The expression of USP39 protein in SMMC-7721/NC, SMMC-7721/KD and SMMC-7721/KD# cells was examined by western blot analysis using GAPDH as an internal control. (C) Overexpression efficiency of USP39 in SMMC-7721 cells was detected by western blot analysis using GAPDH as an internal control. *P<0.05, **P<0.01. USP39, ubiquitin specific peptidase 39.