Fig. 3.

ANP stimulates NPR-A down-regulation in 293T cells overexpressing NPR-A. Cells were incubated with 10 μg/ml cycloheximide and with or without 1 μm ANP for indicated periods of time. ANP was refreshed every 4 h. Top, NPR-A levels were detected by sequential immunoprecipitation-immunoblotting as described in Fig. 1. Bottom, Data from the immunoblot were quantitated and presented in graphical form as a mean and range, where n = 2. Error bars are within the symbols. 293T NPR-A data are representative of three similar experiments.