FIGURE 8.

Liver recovery due to vorinostat treatment is independent of changes in expression of Npc1 or Npc2. Animals were treated with either vehicle (PEG/DMSO) or 150 mg/kg vorinostat from P21 to P60. a, liver RNA from the indicated animals was assessed for Npc1 gene expression by qRT-PCR relative to Gapdh. All groups are n = 3 with data shown as mean ± S.D. (error bars). b, representative image of 30 μg of hepatic protein extracted from vehicle- and vorinostat-treated animals that was denatured at 4 °C for 30 min, subjected to electrophoresis, transferred onto PVDF membrane, and incubated with NPC1 antibody or actin antibody. c, NPC1 and actin bands were quantified relative to actin using densitometry software (ImageJ). All groups are n = 7 with data shown as mean ± S.E. *, p < 0.05, Student's t test. d, liver RNA from the indicated animals was assessed for NPC2 gene expression by qRT-PCR relative to GAPDH. All groups are n = 3 with data shown as mean ± S.D. (error bars). *, p < 0.05, Student's t test. e, protein from the indicated animals was extracted, treated with glycosidase enzymes, and analyzed via SDS-PAGE/immunoblotting and probed for NPC1 expression.