FIGURE 3.

Acetylation of control heart mitochondria with acetic anhydride mimics the respiratory profile of Akita heart mitochondria. A, control and Akita heart mitochondria were treated with the indicated amounts of Ac₂O and then subjected to Western blotting (WB) analysis using an acetyl-lysine (AcK) antibody. Lipoic acid (LA) content was probed as a loading control as its levels were not affected by Ac₂O treatment. The samples shown are from a single blot and the same exposure. The image was subsequently cropped for clarity of presentation. B, state 3 respiration rates were measured 2 min after addition of 10 μm Ac₂O as described under “Experimental Procedures” (n = 4). C, mitochondria (n = 5) were treated with 10 μm Ac₂O as indicated. After 2.0 min, PDH activity was measured as described under “Experimental Procedures.” D, mitochondria (n = 5) were treated with 10 μm Ac₂O as indicated. After 2.0 min, the radiolabeled pyruvate mixture was added, and pyruvate transport was measured as described under “Experimental Procedures.” Experimental points are from unique mitochondrial preparations, and error bars are the standard deviation. *, p < 0.05, unpaired Student's t test.