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. 2017 Feb 1;292(11):4423–4433. doi: 10.1074/jbc.M116.753509

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Acetylation preferentially inhibits uncoupled mitochondrial respiration. A decrease in the ratio of uncoupled to coupled respiration is indicative of decreased pyruvate uptake. A, control heart mitochondria were treated with 10 μm Ac₂O as indicated, and maximal pyruvate-supported respiration was measured upon addition of ADP (coupled; top) or FCCP (uncoupled; bottom). B, the magnitude of state 3 inhibition induced by 10 μm Ac₂O was quantified in coupled and uncoupled mitochondria (n = 4). *, p < 0.05, paired Student's t test. C, the ratio of uncoupled to coupled state 3 respiration was quantified in control heart mitochondria, control heart mitochondria treated with 1.0 μm CHC, control heart mitochondria treated with 10 μm Ac₂O Ac₂O, or Akita heart mitochondria (n > 4). *, p < 0.05, unpaired Student's t test. Error bars are the standard deviation.