FIGURE 5.

Metabolic turnover analysis of Epi WAT in DIO mice. Four-hour-fasted 12-week-old male mice were administered [¹³C₆]glucose (0.2 mg/μl in saline, 1 mg/g of body weight) intraperitoneally. DIO group mice were fed a high-fat/high-sucrose diet from 8–12 weeks of age, whereas control group mice were fed regular diet (n = 6/each group). A, changes in plasma glucose levels. B, changes in plasma insulin levels. C, [¹³C₆]glucose-derived constitutive metabolites of the TCA cycle, pyruvate and glutamate, in Epi WAT. The level of each M+2 isotopomer in control mice was set at 1. Data are mean ± S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.001; control mice versus DIO mice at the same time after [¹³C₆]glucose administration.