FIGURE 4.

SDS-PAGE of purified human recombinant PPIP5Ks. Shown is the analysis by SDS-PAGE and Coomassie Brilliant Blue staining of 5-μl aliquots of each of the indicated recombinant PPIP5Ks after immunoaffinity purification. There are six lanes toward the left-hand side of the gel that are labeled with the quantities of the recombinant human PPIP5K2 kinase domain (39) that were used as standards to calibrate through densitometry the concentrations of the PPIP5K constructs. The lanes containing PPIP5K1 serve as “no-transfection” controls for PPIP5K2 and vice versa.