FIGURE 8.
The effects of varying [Pi] (0–5 mm) upon the 5-InsP7 kinase activities of PPIP5K1 and PPIP5K2. Kinase assays, performed as described under “Experimental Procedures,” contained 1 μm 5-[3H]InsP7, the indicated concentrations of Pi, and either PPIP5K1R399A (gray circles) or PPIP5K2R388A (black circles).Data points represent means and error bars represent S.E. from three biological replicates.