Skip to main content
. 2017 Feb 2;292(11):4556–4570. doi: 10.1074/jbc.M116.768853

FIGURE 2.

FIGURE 2.

Novel SNIPER(ER) with potent protein knockdown activity. A, chemical structure of novel SNIPER(ER). B, protein knockdown activity of SNIPER(ER)-87. MCF-7 cells were treated with the indicated concentrations of SNIPER(ER)-87 for 6 h. Whole-cell lysates were analyzed by Western blotting with the indicated antibodies. Numbers below the ERα panel represent ERα/actin ratio normalized by vehicle control as 100. Data in the bar graph are the mean ± S.D. of three independent experiments; asterisks indicate p < 0.05 compared with vehicle control. C, SNIPER(ER)-87 or -88 rapidly down-regulates ERα protein levels. MCF-7 cells were treated with the indicated concentrations of SNIPER(ER)s for the indicated periods. Whole-cell lysates were analyzed by Western blotting with the indicated antibodies. Numbers below the ERα panel represent the ERα/actin ratio normalized by the vehicle control as 100. D, optimization of linker length in the SNIPER(ER). MCF-7 cells were treated with the indicated concentrations of SNIPER(ER)s or a mixture of the ligands for 24 h and then analyzed by Western blotting. E, SNIPER(ER)-87 degrades ERα in human breast tumor T47D and ZR75-1 cells.