FIGURE 8.

HNF-1β overexpression protects renal tubular cells from cisplatin injury. RPTC cells were transfected with Myc-HNF-1β or empty vector. The cells were then treated with 20 μm cisplatin for 16 h. A, representative images of cell morphology (phase-contrast) and nuclear staining to show apoptosis. B, percentage of apoptosis induced by cisplatin in HNF-1β or empty vector-transfected cells. *, statistically significant different from vector group (p < 0.05, n = 3). C, immunoblot analysis of PARP and caspase 3 cleavage during cisplatin treatment of HNF-1β or empty vector-transfected cells. D, immunoblot analysis of HNF-1β and Myc tag in Myc-HNF-1β or empty vector transfected cells. Cyclophilin B was used as internal loading control. E, densitometry quantification of HNF-1β expression after normalization with cyclophilin B (n = 3, *, p < 0.05 versus empty vector-transfected cells).