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NMR spectroscopy of CaM binding to ER(287–305). A, 15N-1H HSQC spectra of 15N-labeled full-length CaM (black), CaM-N (cyan), and CaM-C (magenta) in the presence of unlabeled ER(287–305). B, constant time 13C-1H HSQC spectra of 13C-labeled full-length CaM (black), CaM-N (cyan), and CaM-C (magenta) in the presence of unlabeled ER(287–305). The experimental conditions are defined under “Experimental Procedures” and were the same as described previously (15).
