ST6Gal-I activity promotes the activation of prosurvival molecules upon acute serum deprivation.
A, OV4 cells were serum-deprived for 4, 18, or 24 h and then immunoblotted for pAkt, total Akt, p-p70S6K, and total p70S6K. B, densitometric analyses for pAkt and total Akt for three blots. Values for pAkt and total Akt were each normalized to β-tubulin, and then data were plotted as pAkt/total Akt. *, p < 0.05. C, densitometric analyses for p-p70S6K and total p70S6K for three blots. Values for p-p70S6K and total p70S6K were each normalized to β-tubulin, and then data were plotted as p-p70S6K/total p70S6K. *, p < 0.05.