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. 2017 Jan 30;292(11):4663–4673. doi: 10.1074/jbc.M116.763862

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — ST6Gal-I activity up-regulates the expression of NFκB pathway proteins. A and B, OV4 (A) and BxPC3 (B) cells were grown in 10% or 1% FBS for 24 h and then immunoblotted for pNFκB and total NFκB (p65). C, densitometry for three OV4 immunoblots. Values for pNFκB and total NFκB were each normalized to β-tubulin. pNFκB and total NFκB were graphed separately (rather than phospho/total) because differences were noted in total NFκB levels. *, p < 0.05. D, densitometry for three BxPC3 immunoblots. Values for pNFκB and total NFκB were each normalized to β-tubulin. *, p < 0.05. E and F, OV4 (E) and BxPC3 (F) cells were serum-starved for 6 or 24 h and then analyzed by qRT-PCR for various prosurvival molecules known to be transcriptionally up-regulated by NFκB. Values represent the averages of at least four independent experiments ± S.D., with each independent experiment performed in triplicate. *, p < 0.05.