FIGURE 3.

In vitro hydrolysis of representative substrates by CsBGlu12. A, TLC of cello-oligosaccharides in the absence and presence of enzyme CsBGlu12. G, C2, C3, C4, and C5 depict glucose, cellobiose, cellotriose, cellotetraose, and cellopentose. LC-MS chromatograms of Kaempferol 3-O-β-glucoside in absence and in presence of enzyme (B) and 1-O-sinapoyl-β-d-glucose in absence and in presence of the enzyme CsBGlu12 (C) are shown. Substrates S1, kaempferol 3-O-β-glucoside (m/z 447), and S2, 1-O-sinapoyl-β-d-glucose (m/z 385), were converted to products P1 (m/z 285) and P2 (m/z 223), respectively.