FIGURE 6.

Expression of Csbglu12 and accumulation of unconjugated flavonols. Expression of Csbglu12 was determined using quantitative real time PCR of different tissues (A) and different developmental stages of flower (B) as follows: 4 days before anthesis (−4da; 3 days before anthesis (−3da); 2 days before anthesis (−2da); day of anthesis (da); 2 days after anthesis (+2da); and 3 days after anthesis. C, hormonal treatments as follows: control (Ctrl); MeJ, 2,4-dichlorophenoxyacetic acid (2,4-D); ABA, salicylic acid (SA), and gibberellic acid (GA3). D, stress treatments as follows: sodium chloride (NaCl); ultraviolet-B (UV); methyl viologen (MV); dehydration (Dehyd); and 4 °C (cold). 18S was used as endogenous control. Concentration of flavonols (Kaempferol and Quercetin) was determined in different tissues (E), different developmental stages (F), different hormonal treatments (G), and stress treatments (H). All experiments were carried out in triplicate and expressed as mean ± S.D. Differences between the control and treatments were analyzed by Student's t test and considered statistically significant at *, p < 0.05.