FIGURE 6.
Isoproterenol-induced cAMP accumulation is attenuated in HEK293ΔT2/T3 cells. The WT Myc- and FLAG-tagged hβ1AR was transiently transfected into HEK293ΔT2/T3 or HEK293WT cells for 48 h. Cells were stimulated with increasing concentrations of isoproterenol for 60 min, and cAMP was measured using the Cisbio Bioassays cAMP assay. The data represent the mean ± S.E. (error bars) of three independent experiments performed in duplicate. The values were normalized to the maximum signal obtained for the HEK293WT cells, which was set to 100%.