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. 2017 Feb 8;292(12):4789–4800. doi: 10.1074/jbc.M116.763821

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Phylogenetic tree of EGCase and its homologs in the GH5 family based on the maximum likelihood method with 100 bootstrap replications conducted by MEGA6. The sequence of M750_EGCase I was obtained from the literature (15), whereas the other sequences included in the analysis were obtained from the NCBI database. All bootstrap values are displayed. Scale bar, 0.2 amino acid substitutions/site. The three-dimensional structure of 103S_EGCase I (GenBank^TM accession number CBH49814) from R. equi 103S solved in this study is marked with a red star. M777_EGCase II (GenBank^TM accession number AAB67050) was from Rhodococcus sp. strain M-777, EGALC (GenBank^TM accession number BAF56440) was from R. equi, EGCrP1 (GenBank^TM accession number BAL46040) was from C. neoformans, and EGCrP2 (GenBank^TM accession number AFR99035) was from C. neoformans. The GH5 subfamily number for each branch is shown.