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Kinetic parameters for 103S_EGCase I and M777_EGCase II using GM1 as the substrate
Kinetic assays were performed in triplicate. The fitting curves for the kinetic parameters are presented in supplemental Fig. S3.
| Enzyme | kcat | Km | kcat/Km |
|---|---|---|---|
| min−1 | mm | min−1 mm−1 | |
| 103S_EGCase I | 630 ± 50 | 0.23 ± 0.03 | 2740 |
| M777_EGCase II | 5.3 ± 0.4 | 0.25 ± 0.04 | 21 |
