TABLE 4.

Specific activities of M777_EGCase II and its mutants toward the GM1, LacCer, and fucosyl-GM1 substrates

EGCase II	GM1		LacCer		Fucosyl-GM1
	Specific activity	Relative activity	Specific activity	Relative activity	Specific activity	Relative activity
	nmol/min/μg	%	nmol/min/μg	%	nmol/min/μg	%
WT	0.223 ± 0.012	100	0.250 ± 0.010	100	<0.0001	100
S63G	2.099 ± 0.016	940	0.228 ± 0.015	91.5	0.137 ± 0.008	>137000
Loop deletion (Asn148–Gly154)	0.364 ± 0.009	163	0.117 ± 0.009	46.8	<0.0001	100