FIGURE 7.

Eng1 but not Exg8 affects the macrophage proinflammatory cytokine response to Histoplasma. Macrophage production of TNFα (A) and IL-6 (B) 8 h after infection with wild-type (gfp-RNAi), Exg8-, Eng1-, or Eng1-Exg8 glucanase-deficient yeasts (m.o.i. 0.5:1). Cytokines were measured by ELISA and levels determined by comparison with cytokine standards. Asterisks indicate statistically significant differences between wild-type and glucanase-deficient yeasts as determined by Student's t test (ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001).