TABLE 1.
Enzyme kinetics for Histoplasma Eng1 and Exg8
Steady-state kinetic parameters were determined for purified Eng1 and Exg8 hydrolyses of laminarin substrate. For each, two independent protein purifications were assayed by incubation with laminarin substrate and quantification of the reducing sugars released. Vmax, Km, and Kcat values were determined by curve fitting of the data obtained from two different enzyme concentrations while varying the laminarin substrate concentration.
| Vmax | Km | Kcat | Kcat/Km | |
|---|---|---|---|---|
| units/mg | mg/ml | 1/s | ml/(mg s) | |
| Eng1 | 17.9 ± 1.1 | 1.62 ± 0.38 | 28.2 ± 1.7 | 17.3 ± 4.4 |
| Exg8 | 48.1 ± 2.5 | 1.00 ± 0.24 | 75.8 ± 3.9 | 75.8 ± 16.4 |