TABLE 3.
Strain | Genotypea | Other designation |
---|---|---|
WU8 | (G186A) ura5-32Δ | WT |
OSU22 | (G186A) ura5-32Δ zzz::pCR482 [hph, gfp] | RNAi sentinel |
OSU244 | (G186A) ura5-32Δ zzz::pCR482 [hph, gfp] zzz::pED02 [URA5, gfp-RNAi] | gfp-RNAi |
OSU245 | (G186A) ura5-32Δ zzz::pCR482 [hph, gfp] zzz::pED06 [URA5, gfp:ENG1-RNAi] | ENG1-RNAi |
OSU205 | (G186A) ura5-32Δ zzz::pCR482 [hph, gfp] zzz::pAG26 [URA5, gfp:EXG8-RNAi] | EXG8-RNAi |
OSU214 | (G186A) ura5-32Δ zzz::pCR482 [hph, gfp] zzz::pAG37 [URA5, gfp:ENG1:EXG8-RNAi] | ENG1:EXG8-RNAi |
WU15 | (G217B) ura5-42Δ | WT |
OSU194 | (G217B) ura5-42Δ zzz::pAG21 [G418R, gfp] | RNAi sentinel |
OSU247 | (G217B) ura5-42Δ zzz::pAG21 [G418R, gfp] zzz::pED02 [URA5, gfp-RNAi] | gfp-RNAi |
OSU248 | (G217B) ura5-42Δ zzz::pAG21 [G418R, gfp] zzz::pED06 [URA5, gfp:ENG1-RNAi] | ENG1-RNAi |
OSU273 | (G217B) ura5-42Δ zzz::pAG21 [G418R, gfp] zzz:pAG26 [URA5, gfp:EXG8-RNAi] | EXG8-RNAi |
OSU275 | (G217B) ura5-42Δ zzz::pAG21 [G418R, gfp] zzz:pAG37 [URA5, gfp:ENG1:EXG8-RNAi] | ENG1:EXG8-RNAi |
OSU265 | (G217B) ura5-42Δ zzz:pQS14 [URA5, PH2B-ENG1:FLAG] | ENG1:FLAG |
OSU266 | (G217B) ura5-42Δ zzz:pAG39 [URA5, PH2B-ENG1:6xHIS] | ENG1:6xHIS |
OSU313 | (G217B) ura5-42Δ zzz::pKD02 [URA5, PH2B-EXG8:FLAG] | EXG8:FLAG |
OSU314 | (G186A) ura5-32Δ zzz::pKD01 [URA5, PH2B-EXG8:6xHIS] | EXG8: HIS6 |
OSU328 | (G217B) ura5-42Δ pmt2::T-DNA [hph] zzz:pQS14 [URA5, PH2B-ENG1:FLAG] | pmt2;ENG1:FLAG |
OSU329 | (G217B) ura5-42Δ pmt2::T-DNA [hph] zzz:pKD02 [URA5, PH2B-EXG8:FLAG] | pmt2;EXG8:FLAG |
a Strains were constructed in the G186A (ATCC no. 26029) or G217B (ATCC no. 26032) Histoplasma strain backgrounds.