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. 2017 Feb 8;292(12):4847–4860. doi: 10.1074/jbc.M116.767541

FIGURE 2.

FIGURE 2.

Binding of GFP-CBMs to Sigmacell 20 cellulose powder. The average percentages of the different amounts of GFP-CBM (10–100 pmol) contacted with 5 mg of Sigmacell 20 that bound to the substrate after 20 min of incubation are shown. The extremes of the thinner bars superimposed on each data point correspond to the minimum and maximum percentages of adsorption measured across the range of loaded amounts of GFP-CBM. Triplicate experiments were performed in 100 mm phosphate buffer, pH 7, 25 °C.