TABLE 2.

The effects of inhibiting substrate oxidation to different levels on the ratio of ATP production to substrate oxidation

ATP synthesis was carried out using SMPs as described under “Experimental Procedures,” and the substrate oxidation reactions were inhibited as in Table I but using three different inhibitor concentrations for each reaction. The values in bold correspond to the inhibitor concentrations used in Table I and for stoichiometry determination.

	NADH:O2			Succinate:O2			NADH:Q1
Inhibition of substrate oxidation (%)	61.3 ± 4.9	69.7 ± 1.6	84.0 ± 1.3	38.2 ± 1.8	52.7 ± 2.6	67.1 ± 0.3	0.0 ± 6.0	13.7 ± 2.7	30.4 ± 0.6
ATP/2 e−	1.29 ± 0.19	1.35 ± 0.08	1.35 ± 0.11	0.79 ± 0.04	0.81 ± 0.05	0.83 ± 0.01	0.54 ± 0.04	0.53 ± 0.02	0.55 ± 0.01