TABLE 3.
Complex I proton pumping stoichiometry measurements in SMPs
The results from four independent complex I stoichiometry measurements in B. taurus SMPs, each with errors propagated from the standard errors in the rate measurements, are given. In the last column the efficiency (which is equal to (1 − kLeak) × 100%) is reported as the average of the values from the three reactions. The bottom row displays the averages from all four measurements, with propagated errors.
| Stoichiometry from comparison of pairs of reactions |
Efficiency of ATP synthesis assuming 4 H+/2 e− for complex I | |||
|---|---|---|---|---|
| NADH:O2 vs. succinate:O2 | NADH:Q1 vs. succinate:O2 | NADH:O2 vs. NADH:Q1 | ||
| Experiment 1 | 4.39 ± 0.09 | 4.13 ± 0.07 | 3.95 ± 0.08 | 35.6 ± 0.4 |
| Experiment 2 | 3.78 ± 0.11 | 3.63 ± 0.08 | 3.54 ± 0.09 | 42.5 ± 1.2 |
| Experiment 3 | 4.24 ± 0.16 | 4.23 ± 0.12 | 4.23 ± 0.16 | 38.3 ± 0.6 |
| Experiment 4 | 4.42 ± 0.06 | 4.41 ± 0.16 | 4.40 ± 0.17 | 40.3 ± 1.1 |
| Average | 4.21 ± 0.15 | 4.10 ± 0.17 | 4.02 ± 0.19 | 39.2 ± 3.0 |