FIGURE 5.

Formation of STI-trapped intasomes with IN octamers requires viral DNA and STI. RSV IN(1–278) was used with 18R GU3 in the presence (black line) and absence (w/o STI) (blue line) of MK-2048 to assemble intasomes under standard conditions at 18 °C for 48 h. A parallel assembly with MK-2048 using a 21R-NSP ODN with nonspecific DNA sequences (red line) was also analyzed. Elution volume (ml) and absorbance at 280 nm are indicated. AU, arbitrary units.