FIGURE 3.
Reversing the side chain charge in positions Glu-103 or Arg-131 changes the potency and efficacy of glycine. A, representative whole cell current responses to glycine applied to GlyR α1 mutants. B, clusters of single-channel mutant GlyR activity elicited in cell-attached patches by saturating concentrations of glycine. C, whole cell concentration-response curves for the effect of glycine on E103A, E103R, R131A, and R131E mutant GlyR (n = 3, 8, 5, and 4, respectively). Curves are scaled to the maximum Popen obtained from single channel recordings. The WT concentration-response curve for glycine is shown for comparison (solid line; the data for this curve is shown in Fig. 2C).