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. 2017 Feb 6;292(12):5043–5054. doi: 10.1074/jbc.M116.771014

FIGURE 3.

FIGURE 3.

Suppression of vorinostat-induced IL-8/CXCL8 expression enhances vorinostat proapoptotic and antiproliferative effects in OC cells. A and B, SKOV3 cells were transfected with control siRNA or IL-8-specific siRNA-1 or siRNA-2, treated for 48 h with 0, 2, and 5 μm vorinostat, and analyzed for IL-8 mRNA expression by real-time RT-PCR (A) and for IL-8 release by ELISA (B). C, cell viability was measured by trypan blue exclusion in cells transfected with control, IL-8 siRNA-1, or IL-8 siRNA-2 and incubated with vorinostat for 48 h. D and E, apoptosis was analyzed by cytoplasmic nucleosome enrichment assay (D), and the colony formation rate was evaluated as the percentage of plated cells that formed colonies (E) in cells transfected with control or IL-8 siRNA-1 and incubated with 0, 2, and 5 μm vorinostat for 48 h. F, cell proliferation was measured by CellTiter 96 One Solution cell proliferation assay in cells transfected with control or IL-8 siRNA-1 and incubated with 1.5 μm vorinostat or control DMSO. The values represent the mean ± S.E. of four experiments. *, p < 0.05; **, p < 0.01 compared with cells transfected with the corresponding control siRNA.