FIGURE 4.

Neutralization of vorinostat-induced IL-8/CXCL8 potentiates the vorinostat inhibitory effect on OC cell viability and proliferation. A and B, SKOV3 and CAOV3 cells were incubated for 48 h with 0, 2, and 5 μm vorinostat in the presence of 2 μg/ml of control mouse IgG1 or 2 μg/ml of mouse anti-human IL-8 IgG1, and cell viability (A) and proliferation (B) were measured as described above. C, Western blotting analysis of CXCR1 and CXCR2 in WCEs of SKOV3 cells incubated with 0, 2, and 5 μm vorinostat for 48 h. Each lane corresponds to ∼5 × 10⁴ cells. D, proliferation of SKOV3 cells incubated for 48 h with 0 and 5 μm vorinostat in the presence of 2 μg/ml of control mouse IgG1 or anti-IL-8 IgG1 with and without 0.5 μg/ml of exogenously added recombinant human IL-8/CXCL8 or CXCL5 proteins. The values represent the mean ± S.E. of three experiments. *, p < 0.05; **, p < 0.01 compared with cells incubated with control mouse IgG1.