FIGURE 6.

TG species distribution in SMS1 and control cells. HepG2 cells were cultured for 18 h in the presence of either 0.5 mm BSA as a vehicle control (upper panel) or 1 mm palmitic acid delivered as a BSA complex (2:1, mol/mol) (lower panel) for 18 h. TG fatty acid composition measured by mass spectrometry as described under “Experimental Procedures.” The graphs represent S-plots of OPLS-DA of TG fatty acid composition and indicate the major features that contributed to the group difference between HepG2-EV and HepG2-SMS1 in each case. Data are the average of three replicates.