FIGURE 8.
Effect of SMS1 overexpression on the de novo synthesis of PC. HepG2-SMS1 and EV control cells were cultured in complete growth medium supplemented with 0.3 μCi/well radiolabeled [14C]choline chloride for the indicated periods of time. Lipids were extracted and separated by TLC as described under “Experimental Procedures.” Radioactivity from the corresponding bands was quantified by scintillation counting. A, phosphatidylcholine; B, sphingomyelin. Data shown are the average ± S.D. (error bars), n = 3 (*, p < 0.05). Results were confirmed in two independent experiments.