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. 2017 Jan 13;292(12):5110–5122. doi: 10.1074/jbc.M116.751602

TABLE 1.

Effect of SMS1 overexpression on TAG fatty acid composition in control conditions and in the presence of 1 mm palmitic acid

The identification of each TG lipid species is based on exact monoisotopic precursor mass, fragment ion information, and theoretical isotopic distribution.

m/za Accepted identificationb Total carbon: double bond 1 mm palmitic acid, HepG2-SMS1 versus HepG2-EV
Vehicle, HepG2-SMS1 versus HepG2-EV
-Fold change p(corr) -Fold change p(corr)
970.7855 TG (18:3/20:3/22:5) TG (60:11) 11.1 0.997344 3.8 0.968865
972.8017 TG (18:1/20:3/22:6) TG (60:10) 7.2 0.995491 2.5 0.973176
944.7698 TG (18:3/18:3/22:4) TG (58:10) 6.2 0.996561 2.4 0.963495
948.8008 TG (18:1/18:4/22:3) TG (58:8) 4.5 0.994698 1.6 0.941047
924.8014 TG (16:0/18:1/22:5) TG (56:6) 4.2 0.997911 2.1 0.957018
898.7848 TG (16:0/18:1/20:4) TG (54:5) 3.8 0.997447 2.1 0.955878
894.7545 TG (16:0/18:3:20:4) TG (54:7) NDc ND 1.7 0.957635
926.8156 TG (16:0/18:1/18:2) TG (52:3) 2.9 0.989006 1.5 0.919795
950.8175 TG (18:3/20:1/20:3) TG (58:7) 2.7 0.986254 1.7 0.944278
948.8008 TG (18:1/18:4/22:3) TG (58:8) ND ND 1.7 0.941047
922.7857 TG (16:0/18:1/22:6) TG (56:7) 2.6 0.985647 1.4 0.917071
952.8328 TG (18:0/18:2/22:4) TG (58:6) 2.6 0.992549 1.6 0.931402
874.7859 TG (16:1/18:0/22:4) TG (56:5) 2.3 0.995110 ND ND
902.8155 TG (16:0/18:0/20:3) TG (54:3) 2.2 0.973230 1.1 0.801826
926.8173 TG (16:0/20:1/20:4) TG (56:5) 2.1 0.983169 2.0 0.919795
870.7542 TG (16:0/18:2/18:3) TG (52:5) 2.0 0.989413 1.8 0.949937
896.7698 TG (16:0/16:1/22:5) TG (54:6) 2.0 0.954632 1.4 0.878744
896.7707 TG (16:0/18:1/20:5) TG (54:6) 2.0 0.982693 1.7 0.96172
876.7998 TG (16:0/18:0/18:2) TG (54:2) 1.8 0.940230 1.2 0.804526
872.7705 TG (16:0/16:0/20:4) TG (52:4) 1.7 0.973257 1.2 0.871994
824.7703 TG (16:0/16:0/16:0) TG (48:0) 1.2 0.831157 ND ND
850.7859 TG (16:0/16:0/18:1) TG (50:1) 0.90 −0.648602 1.4 0.883765
822.7545 TG (16:0/16:0/16:1) TG (48:1) 0.53 −0.970125 1.2 0.824069
794.7232 TG (14:0/16:0/16:1) TG (46:1) 0.48 −0.984013 ND ND
848.7702 TG (16:0/16:0/18:2) TG (50:2) 0.42 −0.920163 ND ND
820.7388 TG (16:0/16:1/16:1) TG (48:2) 0.27 −0.954241 ND ND

a All TG were identified as ammonium adducts.

b sn-1, sn-2, and sn-3 fatty acid assignments are arbitrary.

c ND, not detected.