TABLE 2.
Lower and upper limits of quantification and sample input and processing volumes for the 4 assays
| Assaya | PCR target (probe type)b | Internal controlc (type) | Sample processing vol (ml) | Linear ranged |
|
|---|---|---|---|---|---|
| LLOQ | ULOQ | ||||
| HPS/CTM v2 | 5′ UTR (TaqMan) | QS (competitive armored RNA) | 500 | 25 | 3.9 × 108 |
| CAP/CTM v2 | 5′ UTR (TaqMan dual probe) | QS (competitive armored RNA) | 500 | 15 | 1.0 × 108 |
| cobas 4800 HCV | 5′ UTR (TaqMan dual probe) | QS (noncompetitive armored RNA) | 500 | 15 | 1.0 × 108 |
| cobas 6800/8800 HCV | 5′ UTR (TaqMan dual probe) | QS (noncompetitive armored RNA) | 500 | 15 | 1.0 × 108 |
a
CAP/CTM v2, Cobas AmpliPrep/Cobas TaqMan HCV quantitative test, version 2; HCV, hepatitis C virus; HPS/CTM v2, Cobas TaqMan HCV test, version 2 for use with the High Pure system.
b
UTR, untranslated region.
c
QS, quantification standard.
d
LLOQ, lower limit of quantification; ULOQ, upper limit of quantification.