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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: Sci Signal. 2016 Oct 18;9(450):rs12. doi: 10.1126/scisignal.aaf5011

Figure 5. shRNA library screen to identify the ALK TKI sensitizers.

Figure 5

(A) Schematic of the shRNA library screen for ALK TKI sensitizers (left), with a representative Venn diagram of shRNAs that sensitized cells to crizotinib, alectinib or both (middle), and representative viability assays for one of these targets, CC2D1A (right). Data represents results from a single experiment, where five unique shRNA were targeted to each gene, and each drug/shRNA effect was assayed in five replicates (B and C) Interactions among the proteins that sensitized cells to crizotinib (B) and alectinib (C) were extracted from the entire EML4-ALK interaction network. The protein types and pTyr change induced by crizotinib are highlighted.