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. 2017 Apr 3;7:45815. doi: 10.1038/srep45815

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Copyright © 2017, The Author(s)

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The genomic H19 G-quadruplex sequence regulates H19 expression. (A,B) Control mESCs (WT-G4 cell) or Mut2-G4-mESCs (Mut-G4-a cell)were cultured in mESC maintenance medium (undifferentiated) or differentiated in EB medium for 6 days (EB Day6) in the presence or absence of L1H1-7OTD (5 μM). The qPCR analyses of H19 RNA levels are shown. (C) qRT-PCR analysis of H19 RNA levels in WT- and Mut-G4-a, or -b cells in EB culture on day 6. (D–F) qRT-PCR analysis for the RNA levels of H19 (D), E2F1 (E) and Sp1 (F) in WT- and Mut2-G4 cells in mECS maintenance medium (undifferentiated) or in SFEBq culture on day 5. (G,H) qRT-PCR analysis for H19 RNA levels in WT- (G) and Mut2-G4 cells (H) transfected with or without the E2F1-coding plasmids. (A–H) Values are normalized against β-actin. mean ± s.d. from three experiments; *P < 0.05, **P < 0.01, ***P < 0.005 analyzed by Dunnett’s multiple-comparison test (A–F) and two-tailed t-test (G,H).