Figure 6.

Transcriptome profiling changes of gonadal development-related genes in ovaries of foxl2a^−/− and foxl2b^−/− mutants. (A) Relatively transcribed changes of several kinds of gonadal development-related genes between foxl2a^−/− and WT ovaries or foxl2b^−/− and WT ovaries at 150 dpf. (B–N) Relative expression qRT-PCR detection of the indicated genes in WT, foxl2a^−/−, and foxl2b^−/− ovaries from 105 to 270 dpf (n = 3). The qRT-PCR quantification of each gene expression is normalized to actb1, and the data are presented as mean ± SEM * P ≤ 0.05 (Duncan’s multiple range test).