Foxl2a and foxl2b cooperatively regulate ovary differentiation and maintenance in zebrafish. (A–V) Relative expression qRT-PCR detection of the indicated genes in ovary (O), ovotestis (O/T), and testis (T) of homozygous double mutants (foxl2a−/−/foxl2b−/−) at 60 dpf (n = 3). Gene names are indicated at the top. (W–Y) Relative expression qRT-PCR detection of (W) foxl2a-1, (X) foxl2a-2, and (Y) foxl2b in the ovaries of WT and foxl2b mutants (foxl2b−/−) or foxl2a mutants (foxl2a−/−) from 35 to 270 dpf, respectively (n = 3). The qRT-PCR quantification of each gene expression is normalized to actb1, and the data are presented as mean ± SEM * P ≤ 0.05 (Duncan’s multiple range test).