Figure 5.

Thue rule recognition. (a) Sequence design of a DNA template encoding a string of 10 symbols separated by spacers. The symbol combinations ec (red), ce (green) and ae (blue) occurred once only within the string, whereas ba (pink) occurred twice. The complementary DNA primers consisted of a clamp sequence followed by the symbol combination and flanking spacers. (b) Capillary electrophoresis analysis (measurement of molecular weight—Bioanalyser 2100, Agilent Technologies) of PCR products for Thue rule recognition. Using the string template DNA described in (a), PCRs were carried out to insert a clamp sequence prior to each Thue rule symbol combination. For primers targeting the symbols ec, ce and ae, only one PCR product is created, while for ba, two products occur.