Table 1.
Homology searches of DAF-16 in the phylum Nematoda for its respective core DNA-binding regions
| Species | Nematode clade | Current annotation | Core DNA binding region | Website/Reference |
|---|---|---|---|---|
| Caenorhabditis elegans | V | O16850 | 222AGWKNSIRHNLSLHSRF238 | Massey et al. (2003) |
| Pristionchus pacificus | V | L0CML2 | AGWKNSIRHNLSLHSRF | Ogawa et al. (2011) |
| Strongyloides stercoralis | IVa | Q6WKW2 | AGWKNSIRHNLSLHNRF | Massey et al. (2003) |
| Meloidogyne hapla | IVb | Contig353.frz3.gene4 | QGWKNSIRHNLSLHSRF | Nematode.net; Opperman et al. (2008) |
| Meloidogyne incognita | IVb | Minc17526 (UPI00060F5D60) | WGWQNSIRHNLSLHDCF | Meloidogyne genomic resources (INRA); Abad et al. (2008) |
| Globodera pallida | IVb | GPLIN_001276900 | SGWKNSVRHNLSLNKCF | Gene DB |
| Heterodera schachtii | IVb | HS00253 | QGWKNSIRHNLSLHSRF | Nematode.net |
| Bursaphelenchus xylophilus | IVb | H2DMI5 | AGWKNSIRHNLSLHSRF | Kikuchi et al. (2011) |
| **:**:******.. * |
The predicted residues involved in direct contact with DNA are highlighted in bold according to a previous analysis of the forkhead DNA-binding domain in DAF-16 (Obsil and Obsilova, 2011; Nakagawa et al., 2013). Protein BLAST searches were performed using C. elegans DAF-16 proteins as query sequences. Multiple amino acid alignments were performed with Clustal Omega (Sievers et al., 2011). The consensus symbols refer to fully conserved (*), strongly similar (:) and weakly similar (.) sequences. ‘Nematode clade’ refers to the five major phylogenetic groups within nematodes according to Blaxter (1998).