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. 2017 Jan 12;119(5):775–789. doi: 10.1093/aob/mcw260

Table 1.

Homology searches of DAF-16 in the phylum Nematoda for its respective core DNA-binding regions

Species Nematode clade Current annotation Core DNA binding region Website/Reference
Caenorhabditis elegans V O16850 222AGWKNSIRHNLSLHSRF238 Massey et al. (2003)
Pristionchus pacificus V L0CML2 AGWKNSIRHNLSLHSRF Ogawa et al. (2011)
Strongyloides stercoralis IVa Q6WKW2 AGWKNSIRHNLSLHNRF Massey et al. (2003)
Meloidogyne hapla IVb Contig353.frz3.gene4 QGWKNSIRHNLSLHSRF Nematode.net; Opperman et al. (2008)
Meloidogyne incognita IVb Minc17526 (UPI00060F5D60) WGWQNSIRHNLSLHDCF Meloidogyne genomic resources (INRA); Abad et al. (2008)
Globodera pallida IVb GPLIN_001276900 SGWKNSVRHNLSLNKCF Gene DB
Heterodera schachtii IVb HS00253 QGWKNSIRHNLSLHSRF Nematode.net
Bursaphelenchus xylophilus IVb H2DMI5 AGWKNSIRHNLSLHSRF Kikuchi et al. (2011)
**:**:******.. *

The predicted residues involved in direct contact with DNA are highlighted in bold according to a previous analysis of the forkhead DNA-binding domain in DAF-16 (Obsil and Obsilova, 2011; Nakagawa et al., 2013). Protein BLAST searches were performed using C. elegans DAF-16 proteins as query sequences. Multiple amino acid alignments were performed with Clustal Omega (Sievers et al., 2011). The consensus symbols refer to fully conserved (*), strongly similar (:) and weakly similar (.) sequences. ‘Nematode clade’ refers to the five major phylogenetic groups within nematodes according to Blaxter (1998).