Fig. 3.

Expression of DR3 on human CD14⁺ monocyte osteoclast precursors.

CD14⁺ monocytes were isolated from pre-menopausal females (n = 7) and cultured on ivory discs for 7 days in media + MCSF. DR3 expression was determined by flow cytometry. a) DR3 was not detected on freshly isolated CD14⁺ monocytes but confirmed after 7 days culture in MCSF (light grey line = isotype, shaded peak = DR3). b) DR3 expression was maintained in cultures following addition of RANKL (black line = isotype, grey line = DR3).