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. 2017 Apr 3;108(3):419–426. doi: 10.1111/cas.13157

Figure 1.

Figure 1

Vasohibin‐2 (VASH2) knockdown reduces the expression of activin receptor‐like kinase 5 (ALK5). (a) Quantitative real‐time RT‐PCR analysis of VASH2, ALK5, and transforming growth factor‐β type II receptor (TβRII) expression in DISS cells transfected with either control or VASH2 siRNA was carried out 24 h after transfection. Values were normalized to the β‐actin mRNA level. (b) Western blotting of ALK5 in DISS cells transfected with either control (si‐Cont) or VASH2 siRNA was carried out 24 h after transfection. β‐Actin in cell lysate was used as a loading control. (c) Quantitative real‐time RT‐PCR analysis of VASH2, ALK5, and TβRII expression in SKOV3 cells transfected with either control or VASH2 siRNA was undertaken 4 days after transfection. Values were normalized to the β‐actin mRNA level. (d) Western blotting of ALK5 in SKOV3 cells transfected with either control (si‐Cont) or VASH2 siRNA was undertaken 4 days after transfection. α‐Tubulin in cell lysates was used as a loading control. (e) Quantitative real‐time RT‐PCR analysis of VASH2, ALK5, and TβRII expression in DISS cells transfected with mock or shVASH2 was carried out. (f) Western blotting of ALK5 in DISS cells transfected with control mock or shVASH2 was carried out. β‐Actin in cell lysates was used as a loading control. The intensity of each band was determined by densitometry. Values indicate the fold change of ALK5 level normalized to β‐actin. (a,c,e) Mean and SDs are shown (*P < 0.05).