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. 2017 Apr 3;108(3):373–379. doi: 10.1111/cas.13151

Figure 2.

Figure 2

POTEF‐AS1 is regulated by androgen in LNCAP cells. (a) RNA sequencing (RNA‐Seq) and ChIP sequencing (ChIP‐Seq) analyses of POTEF‐AS1 in LNCaP and VCaP cells treated with 10 nM 5α‐dihydrotestosterone (DHT) or ethanol (Et) for 24 h. For ChIP‐Seq data18, 19 of androgen receptor (AR), signal ratios compared with input sample are shown as peaks. (b, c) Quantitative RT‐PCR analysis of POTEF‐AS1 levels following 1 μM bicalutamide (Bic) or DMSO treatment and 10 nM siAR or negative control siRNA (siNC) transfection, following Et or 10 nM DHT treatment for 24 h in LNCaP (b) and VCaP (c) cells (n = 3). AR knockdown was also confirmed by quantitative RT‐PCR analysis. Values represent mean ± SD. *P < 0.05; **P < 0.01. (d) POTEF‐AS1 expression in clinical samples. P‐value was calculated by Mann–Whitney U‐test.