Figure 1.

Adsorption of exosomes by the latex beads. Western blot (a) showed different levels of TRPC5, CD63 and MUC1 protein in peripheral blood of representative metastatic breast cancer patients. Western blot (b) showed different β‐actin protein levels in exosomes in cirExo treated with proteinase K alone or combined with Triton X‐100. NS, no significant versus cirExo; *P < 0.05 versus cirExo. Immunofluorescent staining (c) showed different fluorescence intensity when detecting CD63, MUC1 and TRPC5 in exosomes from plasma, while no fluorescence was observed in the absence of exosomes or primary antibodies.