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. 2017 Apr 3;108(3):380–389. doi: 10.1111/cas.13153

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Identification of FKBP51‐interacting proteins. (a) MCF7 cell lysates were immunoprecipitated with normal rabbit IgG or anti‐FKBP51 antibody. The immunoprecipitates were subjected to SDS‐PAGE and visualized by CBB staining. The protein bands specific to FKBP51 were analyzed with LC/MS/MS. In addition to FKBP51, other proteins were identified. (b) MS/MS product ion spectra obtained by nanoflow LC/MS/MS of immunoprecipitated protein complexes from MCF7 cells using an anti‐FKBP51 antibody. (c) MCF7 cell lysates were immunoprecipitated (IP) with rabbit IgG, mouse IgG, anti‐FKBP51, or anti‐DLC2. Total cell lysates and immunoprecipitates were analyzed using anti‐FKBP51 or anti‐DLC2 antibodies.