Skip to main content
. 2017 Apr 3;108(3):380–389. doi: 10.1111/cas.13153

Figure 3.

Figure 3

Cell motility and invasion assay following overexpression of FKBP51. (a) U2OS cells were transfected with FLAG‐FKBP51 or the FLAG‐mock expression vector for 24 h. Next, proliferation was measured using the WST‐1 assay. Relative fluorescence units (RFU) indicate the relative amount of proliferation. Column graphs show the mean ± SEM from six samples. (b) U2OS cells were transfected with FLAG‐FKBP51 or the FLAG‐mock expression vector for 24 h and then subjected to the wound‐healing assay. Phase contrast images are shown for 0 and 24 h. The dotted lines indicate cells at the start of the experiment, and white lines show the tips of migrated cells after 24 h. (c) Column graphs show the mean ± SEM from three samples. (d) U2OS cells were transfected with FLAG‐FKBP51 for 24 h and then treated with the ROCK inhibitor Y‐27632 (50 μM). After the 24‐h inhibitor treatment, cells were subjected to the wound‐healing assay. Phase contrast images are shown for time points 0 and 5 h. The dotted lines indicate cells at the start of the experiment, and the white lines show the tips of migrated cells after 5 h. Column graphs show the mean ± SEM from four samples. (e) U2OS cells were transfected with FLAG‐FKBP51 or the FLAG‐mock expression vector for 24 h and then subjected to the cell invasion assay. Relative fluorescence units (RFU) indicate the relative amount of proliferation. Column graphs show the mean ± SEM from three samples.