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. 2017 Apr 3;108(3):380–389. doi: 10.1111/cas.13153

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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ROCK activity in FLAG‐FKBP51‐expressing U2OS cells. (a) U2OS cells were transfected with FLAG‐FKBP51 or the FLAG‐mock expression vector for 24 h. Next, cells were immunostained with anti‐cortactin antibody (green) and phalloidin‐ATTO565 (red). Nuclei were stained with Hoechst 33258 (blue). (b) The immunoblot analysis of ROCK1 in U2OS cells treated with FLAG‐FKBP51 or the FLAG vector (left panel), and the relative ROCK activity with or without the ROCK inhibitor Y27632 (10 μM) or ATP (125 μM) (n = 3) (right panel).