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. 2017 Apr 3;108(3):488–496. doi: 10.1111/cas.13160

Figure 3.

Figure 3

Treatment with TOPK inhibitor shows marked growth‐suppressive activity against SCLC cells. (a) Western blot analyses were performed to measure TOPK protein levels in adherent and suspension SCLC cells, 48 h after treatment with OTS514 (0, 10, and 20 nM). MTT assay was performed in six adherent SCLC cells (b) or in five suspension SCLC cells (c). All cells were measured their viability after treatment with OTS514 at different concentrations (0–50 nM) for 72 h. Graphs indicate relative cell viability at each OTS514 concentration, compared to a negative control (untreated). (d) The cellular effects induced by OTS514 treatment (10 nM or 20 nM) in three adherent SCLC cells and two NFLF cells were evaluated at 72 h after treatment by microscopic observation (×200 magnification). Scale bar indicates 50 μm.